Chapter
12
1. Which of the following
can act as a vector to introduce new genes into a cell?
a) human insulin
b) BST
c) bacterial plasmid
d) restriction enzymes
e) none of the above
2. Of these steps,
which one occurs earliest in the process of producing recombinant DNA
for industrial production of human insulin?
a) Human DNA fragments
are mixed with the cut plasmids.
b) The same restriction enzyme is used to isolate the insulin gene
and to cut the plasmid DNA
c) The plasmid containing the gene of interest is reintroduced into the
bacterium
d) bacteria producing insulin are grown in fermentation tanks
3. As of 2003, ____ of the human genome's protein-coding regions had been
sequenced.
a) 100%
b) 95%
c) 90%
d) 75%
4. Which of these statements can be logically inferred from the amount
of DNA shared by chimpanzees and humans?
a) humans evolved from chimpanzees
b) humans and chimpanzees share a relatively recent common ancestor
c) humans are unique and different from all other life forms
d) humans have many more genes than chimpanzees do
e) humans are a more complex life form than chimpanzees
4. We discussed three
applications of recombinant DNA technology: genetically modified foods,
industrial manufacturing of particular products, and gene therapy. Choose
one of these applications, give a concrete example, and explain briefly
how it works (i.e., the steps used in the process). Discuss the arguments
for and against using recombinant DNA technology for this particular
application. Which argument do you support and why?
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Chapter
13
1.
In the Lake Erie water snakes, the frequency of the banded allele is
higher on the mainland than in the island populations. We learned in
class that this difference between the two populations is most likely
due to ___________ because unbanded snakes survive better on the islands
than the banded snakes.
a) diversifying selection
b) stabilizing selection
c) directional selection
d) sexual selection
5. The following statement
is false. Rewrite it so that it is correct.
In the example of evolution by natural selection in marine iguanas we discussed
in class, the marine iguanas developed thicker tails and webbed feet because
they needed these traits to search for food in the water.
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DNA Fingerprinting Lab
1. Cutting DNA with
a particular restriction enzyme produces ___________ that can be separated
by gel electrophoresis.
a) restriction fragments
b) enzymes
c) recombinant DNA
d) plasmids
2. In gel electrophoresis,
a 1000-bp length of DNA will travel ________ than a 5000 bp-length of
DNA.
a) faster
b) slower
c) the same rate as
d) not enough information to determine
3. We used DNA fingerprinting
in a simulation to determine which one of 5 suspects left their DNA at
a crime scene. List and explain two applications of DNA fingerprinting
technology other than forensics.
4. Explain the purpose
of the size marker DNA we used in the DNA fingerprinting exercise.
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